From Crime Scene to Court: How Should Future Research Address the Current Limitations in Forensic Entomology?


  • Dr Kate M Barnes University of Derby
  • Dr Mark T Bulling
  • Dr Ian S Baker
  • Adam S Long


Insect, Post Mortem Interval, Standard Procedure, Validation, Entomological Evidence


Over the last 50 years there has been a dramatic increase in the amount of research being conducted in forensic entomology. Given the gravity of the consequences associated with court decisions, it is essential to assess how research in forensic entomology is supporting its practical application in criminal investigations and in court. In this study we employed a questionnaire-based approach, gathering information from professionals from across the world involved in the collection and preparation of forensic entomological evidence, as well as those who present and utilise such evidence in court. This was supported by a bibliometric approach that examined patterns of research publication and collaboration. The questionnaire survey indicated that forensic entomology is predominantly utilised to determine minimum post-mortem intervals. Whilst entomologists are generally confident in their ability to collect, preserve, store, identify and interpret entomological evidence, a number of weaknesses in the application and use of forensic entomology were identified. Additionally, the bibliometric study revealed extensive clustering of research within countries, with relatively little cross-country collaboration. This may result in inhibited flow of research findings, which is likely exacerbated by the low number of appropriate open-access journals. The vast array of factors that may affect insect behaviour, development, community structure and dynamics, as well as sampling reliability, make forensic entomology particularly challenging. Therefore, standardisation of procedures, validation of methodologies and accreditation frameworks will be critical for maximising the utility of practical forensic entomology. Research must underpin the future development of forensic entomology, but it will be most effective with greater levels of collaboration between research teams, and between researchers and practitioners. The global research community will need to be much more coordinated and standardised if it is to achieve its potential in supporting practical applications inside and outside of the court, thereby maximising the societal value of forensic entomology.


Gennard D. Forensic Entomology: an introduction. Second ed. Wiley-Blackwell; 2012. 184–199.

Benecke M. A brief history of forensic entomology. Forensic Science International. 2001;120(1–2):2–14.

Amendt J, Richards CS, Campobasso CP, Zehner R, Hall MJR. Forensic entomology: Applications and limitations. Forensic Science, Medicine, and Pathology. 2011;7(4):379–92.

Anderson GS. Determining time of death using blow fly eggs in the early postmortem interval. International Journal of Legal Medicine. 2004;118(4):240–1.

Oliveira-Costa J, Antunes de Mello-Patiu C. Application of forensic entomology to estimate the postmortem interval ( PMI ) in homicide investigations by the Rio de Janeiro Police Department in Brazil. Aggrawal’s Internet Journal of Forensic Medicine and Toxicology. 2004;5(1):40–4.

Cherix D, Wyss C, Pape T. Occurrences of flesh flies (Diptera: Sarcophagidae) on human cadavers in Switzerland, and their importance as forensic indicators. Forensic Science International. 2012;220(1–3):158–63.

Goff, M., Flynn M. Determination of post-mortem interval by arthropod succession: A case study from the Hawaiian islands. Journal of Forensic Sciences. 1991;36(2):607–14.

Turchetto M, Lafisca S, Costantini G. Postmortem interval (PMI) determined by study sarcophagous biocenoses: Three cases from the province of Venice (Italy). Forensic Science International. 2001;120(1–2):28–31.

Lefebvre F, Gaudry E. Forensic entomology: A new hypothesis for the chronological succession pattern of necrophagous insect on human corpses. Annales de la Societe Entomologique de France. 2009;45(3):377–92.

Introna F, De Donno A, Santoro V, Corrado S, Romano V, Porcelli F, et al. The bodies of two missing children in an enclosed underground environment. Forensic Science International. 2011;207(1–3):e40–7.

Fremdt H, Amendt J. Species composition of forensically important blow flies (Diptera: Calliphoridae) and flesh flies (Diptera: Sarcophagidae) through space and time. Forensic Science International [Internet]. 2014;236:1–9. Available from:

Matuszewski S, Szafałowicz M, Jarmusz M. Insects colonising carcasses in open and forest habitats of Central Europe: Search for indicators of corpse relocation. Forensic Science International. 2013;231(1–3):234–9.

Picard CJ, Wells JD. A Test for Carrion Fly Full Siblings: A Tool for Detecting Postmortem Relocation of a Corpse. Journal of Forensic Sciences. 2012;57(2):535–8.

Wells J, Introna F, di Vella G, Campobasso C, Hayes J, Sperling F. Human and insect mitochondrial DNA analysis from maggots. Journal of Forensic Sciences. 2001;46(3):685–7.

de Lourdes Chávez-Briones M, Hernández-Cortés R, Díaz-Torres P, Niderhauser-García A, Ancer-Rodríguez J, Jaramillo-Rangel G, et al. Identification of Human Remains by DNA Analysis of the Gastrointestinal Contents of Fly Larvae. Journal of Forensic Sciences. 2013;58(1):248–50.

Zehner R, Amendt J, Krettek R. STR Typing of Human DNA from Fly Larvae Fed on Decomposing Bodies. Journal of Forensic Sciences. 2004;49(2).

Introna F, Campobasso C Pietro, Goff ML, Pietro C, Lee M. Entomotoxicology. Forensic Science International. 2001;120:42–7.

Gosselin M, Wille SMR, Fernandez M del MR, Di Fazio V, Samyn N, De Boeck G, et al. Entomotoxicology, experimental set-up and interpretation for forensic toxicologists. Forensic Science International. 2011;208(1–3):1–9.

George KA, Archer MS, Toop T. Abiotic environmental factors influencing blowfly colonisation patterns in the field. Forensic Science International. 2013;229(1–3):100–7.

Gennard D. Forensic Entomology: an introduction. Wiley; 2007.

Ody H, Bulling MT, Barnes KM. Effects of environmental temperature on oviposition behavior in three blow fly species of forensic importance. Forensic Science International. 2017;275:138–43.

Lutz L, Verhoff MA, Amendt J. Environmental factors influencing flight activity of forensically important female blow flies in Central Europe. International Journal of Legal Medicine. 2019;133(4):1267–78.

Erzinçllioğlu YZ. Areas of Research in Forensic Entomology. Medicine, Science and the Law. 1986;26(4):273–8.

Faucherre, J., Cherix, D., Wyss C. Behavior of Calliphora vicina under extreme conditions. Journal of Insect Behavior. 1999;12:687–69.

George KA, Archer MS, Toop T. Nocturnal Colonization Behavior of Blowflies (Diptera: Calliphoridae) in Southeastern Australia. Journal of Forensic Sciences. 2013;58(SUPPL. 1):112–6.

Voss, S.C., Forbes, S.L., Dadour IR. Decomposition and insect succession on cadavers inside a vehicle environment. Forensic Science, Medicine, and Pathology. 2008;4:22–32.

Goff ML. Comparison of insect species associated with decomposing remains recovered inside dwellings and outdoors on the Island of Oahu, Hawaii. Journal of Forensic Sciences. 1991;36:748–753.

Pohjoismäki JLO, Karhunen PJ, Goebeler S, Saukko P, Sääksjärvi IE. Indoors forensic entomology: Colonization of human remains in closed environments by specific species of sarcosaprophagous flies. Forensic Science International. 2010;199(1–3):38–42.

Reibe S, Madea B. How promptly do blowflies colonise fresh carcasses? A study comparing indoor with outdoor locations. Forensic Science International. 2010;195(1–3):52–7.

Anderson GS. Comparison of Decomposition Rates and Faunal Colonization of Carrion in Indoor and Outdoor Environments. Journal of Forensic Sciences. 2011;56(1):136–42.

Goff M. Problems in estimation of postmortem interval resulting from wrapping of the corpse: a case study from Hawaii. Journal of Agricultural Entomology. 1992;9(4):237–43.

Bhadra P, Hart AJ, Hall MJR. Factors affecting accessibility to blowflies of bodies disposed in suitcases. Forensic Science International. 2014;239:62–72.

Erzinclioglu YZ. The Entomological Investigation of a Concealed Corpse. Medicine, Science and the Law. 1985;25(3):228–30.

Turner B, Wiltshire P. Experimental validation of forensic evidence: A study of the decomposition of buried pigs in a heavy clay soil. Forensic Science International. 1999;101(2):113–22.

VanLaerhoven, S.L. and Anderson GS. Insect succession on buried carrion in two biogeoclimatic zones of British Columbia. Journal of Forensic Sciences. 1999;44:32–43.

Bourel B, Tournel G, Hédouin V, Gosset D. Entomofauna of buried bodies in northern France. International Journal of Legal Medicine. 2004;118(4):215–20.

Simmons T, Cross PA, Adlam RE, Moffatt C. The influence of insects on decomposition rate in buried and surface remains. Journal of Forensic Sciences. 2010;55(4):889–92.

Gunn A, Bird J. The ability of the blowflies Calliphora vomitoria (Linnaeus), Calliphora vicina (Rob-Desvoidy) and Lucilia sericata (Meigen) (Diptera: Calliphoridae) and the muscid flies Muscina stabulans (Fallén) and Muscina prolapsa (Harris) (Diptera: Muscidae) to colo. Forensic Science International. 2011;207(1–3):198–204.

Balme GR, Denning SS, Cammack JA, Watson DW. Blow flies (Diptera: Calliphoridae) survive burial: Evidence of ascending vertical dispersal. Forensic Science International. 2012;216(1–3):e1–4.

Amendt J, Campobasso CP, Gaudry E, Reiter C, LeBlanc HN, J. R. Hall M. Best practice in forensic entomology - Standards and guidelines. International Journal of Legal Medicine. 2007;121(2):90–104.

Gaudry E, Dourel L. Forensic entomology: Implementing quality assurance for expertise work. International Journal of Legal Medicine. 2013;127(5):1031–7.

Magni P, Guercini S, Leighton A, Dadour I. Forensic entomologists: An evaluation of their status. Journal of Insect Science. 2013;13(78):1–9.

Aria M, Cuccurullo C. bibliometrix: An R-tool for comprehensive science mapping analysis. Journal of Informetrics. 2017 Nov 1;11(4):959–75.

R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. 2020.

Blondel VD, Guillaume JL, Lambiotte R, Lefebvre E. Fast unfolding of communities in large networks. Journal of Statistical Mechanics: Theory and Experiment. 2008;2008(10).

Adams ZJO, Hall MJR. Methods used for the killing and preservation of blowfly larvae, and their effect on post-mortem larval length. Forensic Science International. 2003;138(1–3):50–61.

Brown K, Thorne A, Harvey M. Preservation of Calliphora vicina (Diptera: Calliphoridae) pupae for use in post-mortem interval estimation. Forensic Science International. 2012;223(1–3):176–83.

Szpila K. Key for the identification of European and Mediterranean blowflies (Diptera, Calliphoridae) of medical and veterinary importance - adult flies. In: Gennard D, editor. Forensic Entomology: an introduction. second. Wiley-Blackwell; 2012. p. 77–81.

Szpila K. Key for the identification of third instars of European blowflies (Diptera: Calliphoridae) of forensic importance. In: Amendt J, Goff ML, Campobasso C Pietro, Grassberger M, editors. Current concepts in forensic entomology. Springer International Publishing; 2009. p. 43–56.

Sivell O. Blow flies (Diptera: Calliphoridae, Polleniidae, Rhiniidae). Field Studies Council; 2021.

Erzinclioglu Z. Identification. In: Blowflies. 1996. p. 45–52.

Greenberg B, Kunich JC. Keys to the eggs, larvae, pupae and adults of some forensically important flies. In: Entomology and the Law: Flies as forensic indicators. 2002. p. 54–153.

Disney RHL. Forensic science is not a game. Pest Technology. 2011;5(1):16–22.

Hall MJR. The Relationship between Research and Casework in Forensic Entomology. Insects. 2021;12:174–87.

Lutz L, Verhoff MA, Amendt J. To Be There or Not to Be There, That Is the Question—On the Problem of Delayed Sampling of Entomological Evidence. Insects. 2021;12(2):148.

Martire KA, Kemp RI, Sayle M, Newell BR. On the interpretation of likelihood ratios in forensic science evidence: Presentation formats and the weak evidence effect. Forensic Science International. 2014;240:61–8.

Butcher JB, Moore HE, Day CR, Adam CD, Drijfhout FP. Artificial neural network analysis of hydrocarbon profiles for the ageing of Lucilia sericata for post mortem interval estimation. Forensic Science International. 2013;232(1–3):25–31.

Morgan RM, Levin EA. A crisis for the future of forensic science: Lessons from the UK of the importance of epistemology for funding research and development. Forensic Science International: Synergy. 2019;1:243–52.

Gallagher MB, Sandhu S, Kimsey R. Variation in developmental time for geographically distinct populations of the common green bottle fly, Lucilia sericata (Meigen). Journal of Forensic Sciences. 2010;55(2):438–42.

Tarone AM, Picard CJ, Spiegelman C, Foran DR. Population and temperature effects on Lucilia sericata (Diptera: Calliphoridae) body size and minimum development time. Journal of Medical Entomology. 2011;48(5):1062–8.


— Updated on 2022-02-21

How to Cite

From Crime Scene to Court: How Should Future Research Address the Current Limitations in Forensic Entomology?. (2022). Journal of Forensic Entomology.